Fig. 7.

Hydrogen polysulfide (HS_nH) affected the binding affinity of ScCsoR to Scpdo promoter.

A. ScCsoR exhibited binding to the probe DNA containing bsΙ and bsΠ, even in the presence of competitor DNA. DNA probe (1 nM) was incubated with different amounts of ScCsoR (0, 2.2, 8.8, 15.4 µM). Black arrow indicates the free DNA probe, and red arrow indicates the shifted DNA probe. 50‐fold excess of unlabelled competitor DNA and its mixture with probe DNA were also tested.

B. HS_nH detached ScCsoR from the probe DNA. DNA probe (1 nM) was incubated with different concentrations of ScCsoR (0, 0.5, 1.0, 2.0, 4.0, 8.0, 8.0, 8.0, 8.0 µM). Different amounts of HS_nH (0, 1, 2, 3 mM) were added to the reaction system.

C. LC‐MS/MS analysis of HS_nH‐ and DTT‐treated ScCsoR. Details of the MS data are shown in Figs S3–S5.