Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jul 25;13(6):1793–1806. doi: 10.1111/1751-7915.13609

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 The Authors. Microbial Biotechnology published by Society for Applied Microbiology and John Wiley & Sons Ltd

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

PMC Copyright notice

Fig. 1 — The SEVA 3.1 platform.

A. The SEVA 3.1 vectors are generated by merging: (i) the backbone of the SEVA vectors (grey shade), containing the antibiotic resistance module (purple), the origin of replication module (green), origin of transfer (black) and two transcriptional terminators (dark and light grey) and (ii) the cloning site of the BioBrick vectors (orange shade) comprised by: the BioBrick prefix and suffix (light blue) and a BioBrick (red).

B. Graphical overview of the SevaBrick Assembly. Inserts and backbones are amplified using a core set of standard primers which introduce compatible Bsal sites, allowing the construction of expression vectors with varying degrees of complexity via Golden Gate.