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. 2020 Jul 25;13(6):1793–1806. doi: 10.1111/1751-7915.13609

Fig. 3.

Fig. 3

Convergence of DNA parts into SEVA 3.1 platform. A non‐CDS part is converged using the non‐CDS.Brk‐F and the non‐CDS.Brk‐R primers. The non‐CDS.Brk‐F carries the non‐CDS BioBrick prefix (blue) while the non‐CDS.Brk‐R carries the BioBrick suffix (blue). A CDS part is converged using the CDS.BrK‐F and CDS.Brk‐R primers. The CDS.BrK‐F carries the CDS BioBrick prefix while the CDS.Brk‐R carries the BioBrick suffix and a double stop codon (TAATAA). All primers carry a BsaI recognition site (red) at the 5’‐ or 3’‐end which enables assembly with the amplified with Ev/Pv primers repository vector (pSB1C3). After the assembly the BioBrick suffix and prefix are reconstructed.