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. 2020 Jul 25;13(6):1793–1806. doi: 10.1111/1751-7915.13609

Fig. 6.

Fig. 6

Graphical representation of constructed TUs using SevaBrick Assembly and the standard primers for introduction of short functional genetic elements.

A. Each reporter CDS, amplified from the iGEM registry, was assembled with the constitutive promoter BBa_J23100 and the RBS BBa_B0034.

B. sfGFP was assembled with BBa_J23105 promoter and either the BBa_B0034 RBS or six in silico predicted RBSs (RBS 15–20).

C. sfGFP was assembled with either five short constitutive promoters or three inducible expression systems and the RBS BBa_B0034.

D. P. putida transformed with eight different reporter expression vectors. From right to left: mCherry, gfasPurple, aeBlue, mOrange, amilGFP, amilCP, mRFP and amajLime.