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. 2020 Sep 2;22:421–433. doi: 10.1016/j.omtn.2020.08.039

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© 2020.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Confirmation of Subcellular Localization of circFN1

(A) Quantitative real-time PCR for the abundance of circFN1 and FN1 in SR-Huh7 cells treated with actinomycin D at the indicated time point. (B) Quantitative real-time PCR for the abundance of circFN1 and FN1 in SR-HepG2 cells treated with actinomycin D at the indicated time point. (C) Quantitative real-time PCR for the expression of circFN1 and FN1 mRNA in SR-Huh7 cells treated with or without RNase R. (D) Quantitative real-time PCR for the expression of circFN1 and FN1 mRNA in SR-HepG2 cells treated with or without RNase R. (E) Levels of circFN1 in the nuclear and cytoplasmic fractions of SR-Huh7 cells. (F) Levels of circFN1 in the nuclear and cytoplasmic fractions of SR-HepG2 cells. Data are listed as means ± SD of at least three independent experiments. ∗∗p < 0.01.