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The composition of the simulated digestion fluids used during the INFOGEST digestion assay. Enzymes were added based on predefined activity.
| Oral | Gastric | Intestinal |
|---|---|---|
| SSF x1 | SGF x1 | SIF x1 |
| α-amylase, 75 U mL−1 | Pepsin, 2000 U mL−1 | α-chymotrpysin, 25 U mL−1 and trypsin 100 U mL−1 |
| CaCl2, 0.75 mmol L−1 | CaCl2, 0.075 mmol L−1 | CaCl2, 0.3 mmol L−1 |
| milliQ water | milliQ water | milliQ water |
| pH 7 | pH 3 | pH 7 |
