Figure 4.

Results of Apoptosis and Cycle in A549 Cells

(A) The changes of nucleus of A549 cells stained by Hoechst 33258 with BA, BAD, and BAL of 50 μg/mL were observed under an inverted microscope. (B) Effects of BA, BAD, and BAL of 50 μg/mL on cell death were evidenced by annexin VFITC/PI double staining and FACS analysis. (C) Flow cytometry was performed to examine the effects of the cell cycle with BA, BAD, and BAL of 50 μg/mL in A549. (D) Ratio of G₁/G₂. (E) Frequency distribution bar chart for G₁/G₀, S, and G₂/M. (F) Early apoptosis of BAD and BAL to A549 cells at 48 h. (G) Late apoptosis of BAD and BAL to A549 cells at 48 h. (H) Total apoptosis of BAD and BAL to A549 cells at 48 h. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, dose groups versus control.