Skip to main content
. 2020 Sep 14;22(5):4227–4235. doi: 10.3892/mmr.2020.11505

Figure 3.

Figure 3.

BBR reverses HG-induced effects on cell apoptosis and oxidative stress in Müller cells. PDTC is an NF-κB inhibitor. Müller cells were treated with 5.5 mM glucose; 33.3 mM glucose; 33.3 mM glucose and 20 µM BBR; or 33.3 mM glucose and 100 µM PDTC. Cell apoptosis was detected using. (A) Annexin V-FITC apoptosis detection and (B) Hoechst staining (scale bar, 50 µm). (C) Protein expression was determined using western blotting. (D) MMP was detected using the JC-1 assay. (E) Cytoplasmic and nuclear expression levels of cytochrome c were detected using western blotting. (F) GSH, MDA, SOD and CAT contents were measured in treated Müller cells. (G) ROS levels were measured in treated Müller cells. **P<0.01, ***P<0.001 and ****P<0.0001 vs. the control group. #P<0.05, ##P<0.01 and ###P<0.001 vs. the HG group. BBR, berberine; HG, high glucose; PDTC, pyrrolidine dithiocarbamate; MMP, mitochondrial membrane potential; GSH, glutathione; MDA, malondialdehyde; SOD, superoxide dismutase; CAT, catalase; COXIV, mitochondrial cytochrome c oxidase subunit IV.