Table I.
Primer sequences for reverse transcription-quantitative PCR.
| Gene | Primer sequence (5′→3′) |
|---|---|
| Bax | F: GTGGTTGCCCTCTTCTACTTTG |
| R: CCACAAAGATGGTCACTGTCTG | |
| Collagen I | F: CGGTGGTTATGACTTCAG CTTC |
| R: AGAGGGCTGAGTGGGGAAC | |
| BNP | F: GCAGGCCAGCAGGGTCCACTACAC |
| R: GACCTC GCCATTCCGCTGATTCT | |
| Beclin-1 | F: GACAAATCTAAGGAGTTGCCG |
| R: AGAACTGTGAGGACACCCA | |
| BCL2 | F: AGTTCGGTGGGGTCATGTGTG |
| R: CCAGGTATGCACCCAGAGTG | |
| NOX4 | F: GAACCCAAGTTCCAAGCTCA |
| R: GCA CAAAGGTCCAGAAATCC | |
| NOX1 | F: GGCATCCCTTTACTCTGACCT |
| R: TGCTGCTCGAATATGAATG | |
| NOX2 | F: TGTGCACCACGATGAGGAGAAAGATG |
| R: ATTCTGGTGTTGGGGTGTGACTFGC | |
| Caspase-3 | F: CTTTGCGCCATGCTG AAACT |
| R: TCAAATTCCGTGGCCACTT | |
| MMP9 | F: TCCCCAGAGCGTTACTCG CT |
| R: ACCTGG TTCACCCGGTTGTG | |
| MMP2 | F: GAGTAAGAACAAGAAGACATACATC |
| R: GTAATAAGCACCCTTGAA GAAATAG | |
| ANP | F: GGGTGTGAACCA CGAGAAAT |
| R: ACTGTGGTCATGAGCCCTTC | |
| β-MHC | F: GGGCAA AGGCAAAGCAAAGA |
| R: AAAGTGAGGGTGCGTGGAGC | |
| BGN | F: ACAACAAGCTGTCCCGGGTG |
| R: AGC CCATGGGGCAGAAATCG | |
| TPM1 | F: GCTGGTTGAGGAGGAGTT |
| R: ACTTGTTCGTCACCGTTT | |
| Collagen III | F: GGAGTCGGAGGAATGGGTG |
| R: ATTGCGTCCATCAAAGCCTC | |
| CORIN | F: GTACAG TGCGGTGTCCAACA |
| R: ATCCTGTCAATCCTACCCCC | |
| TGF-β1 | F: CCAACTATTGCTTCAGCTCCA |
| R: GTGTCCAGGCTCCAAATGT | |
| GDF15 | F: CTGCTCTTGCTGCTTCTGCT |
| R: TCGTCCGGGTTGAGTTGG | |
| POSTN | F: AGGAGCCGTGTTTGAGACCAT |
| R: CGGTGAAAGTGGTTT GCTGTTT | |
| GAPDH | F: GGTGCTGAGTATGTCGTGGAGT |
| R: CACAGT CTTCTGAGTGGCAGTG |
F, forward; R, reverse; NOX, NAD(P)H oxidase; BNP, brain natriuretic peptide; MMP, matrix metallopeptidase; ANP, atrial natriuretic peptide; β-MHC, β myosin heavy chain; GDF15, growth differentiation factor 15; TPM1, tropomyosin 1; BGN, biglycan; POSTN, periostin; CORIN, atrial natriuretic peptide-converting enzyme; TGF-β1, transforming growth factor β1.