Skip to main content
. 2020 Sep 9;22(5):4163–4172. doi: 10.3892/mmr.2020.11498

Figure 2.

Figure 2.

Influence of SDF1 on the migration, invasion and angiogenesis of VECs. (A) Expression levels of SDF1 in the cultured supernatants of NPCs were detected by ELISA following 24 h of treatment with various concentrations (0, 10, 50, 100, 200 and 500 µg/ml) of anti-SDF1 antibody. (B) Expression levels of SDF1 in the cultured supernatants of NPCs were detected by ELISA following 0, 12, 24 and 48 h treatment with 100 µg/ml of anti-SDF1 antibody. (C) Tube formation ability of VECs was analyzed by Matrigel tube formation assay, and the total branch length was calculated with ImageJ (magnification, ×100) after VECs were treated with the conditioned media from OE-SDF1 or the control group transfected-NPCs along with anti-SDF1 antibody (100 µg/ml, 24 h) or not. (D and E) Cell migration and invasion abilities were analyzed by Transwell assay with SDF1 overexpressed or non-treated NPCs as a chemokine (magnification, ×100). n=3, *P<0.05, **P<0.01, ***P<0.001. SDF1, stromal cell-derived factor 1; NPCs, nucleus pulposus cells; OE-, overexpression; VECs, vascular endothelial cells.