Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Sep 7;22(5):3777–3784. doi: 10.3892/mmr.2020.11491

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © Jiang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

PMC Copyright notice

Figure 1. — T7E1 assay for knockout efficiency. Each sample consists of three lanes in the agarose gel: The left lane is an DNA marker (M), the middle lane is ‘uncut’ (U), meaning no treatment, and the right lane is ‘cut’ (C), digested by the T7E1 endonuclease. The ‘cut’ lane demonstrated additional lower DNA bands and the decreased bright of the bands compare the ‘uncut’ lane. Diverse intensities of lower bands presumably reflect different degrees of mosaic mutations. PUC19 digested by the T7E1 is used as the positive control. T7E1, T7 endonuclease I.