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. 2020 Aug 24;22(5):3833–3839. doi: 10.3892/mmr.2020.11458

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Copyright: © Xu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Anti-aging molecular mechanisms of ICA may be associated with the regulation of p53/p21, SIRT1/6 and NF-κB signaling in D-gal-induced cell aging. (A) The ratio of p-p53/p53 and p-p21/p21, and protein levels of cav-1, SIRT1 and SIRT6 were determined via western blotting. (B) The effects of D-gal and ICA on the ratio of of p-p53/p53 and p-p21/p21, and protein levels of cav-1 determined via western blotting were semi-quantified. (C) The effects of D-gal and ICA on the protein levels of SIRT1 and SIRT6 determined via western blotting were semi-quantified. (D and E) The protein levels of p65 and p50 in IMR-90 cells treated with ICA and D-gal were assessed via western blotting. GAPDH was used as the internal control. Data are presented as the mean ± standard deviation. *P<0.05, **P<0.01 vs. control group; ^#P<0.05, ^##P<0.01 vs. D-gal group. ICA, icariin; SIRT, sirtuin; NF-κB, nuclear factor κB; cav-1, caveolin-1; D-gal, D-galactose.