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. 2020 Sep 9;22(5):4125–4134. doi: 10.3892/mmr.2020.11500

Figure 2.

Figure 2.

Human anti-TLR4 IgG2 specifically recognizes TLR4 with high affinity. (A) ELISA results. TLR4 was used to coat ELISA plates. The wells were incubated with serial dilutions of human anti-TLR4 IgG2. The specific binding of human anti-TLR4 IgG2 and TLR4 was concentration-dependent. (B) A BLItz System was used to determine the binding affinity of TLR4. TLR4 affinity and kinetics assays yielded five curves with the concentrations of human anti-TLR4 IgG2 ranging from 100–1,600 nM. Equilibrium dissociation constant =8.713×10−10 M with TLR4. (C) Affinity of human anti-TLR4 IgG2 for TLR4 was quantified via flow cytometry analysis. (a) Blank; (b) group treated without human anti-TLR4 IgG2; (c) group treated with human anti-TLR4 IgG2. OD450, optical density at 450 nm; TLR4, Toll-like receptor 4.