Figure 3.

Inhibitory effects of different concentrations of human anti-TLR4 IgG2 on LPS-induced macrophages. Macrophages were preincubated with different concentrations (1, 5 and 10 ng/µl) of human anti-TLR4 IgG2 for 2 h, and then induced with 1 ng/µl LPS for 4 h. mRNA expression levels of (A) TNF-α, (B) IFN-β and (C) IL-6 were determined via reverse transcription-quantitative PCR and normalized to that of the internal control, β-actin. The optimal concentration of human anti-TLR4 IgG2 was 5 ng/µl. All experiments were performed independently ≥3 times. Data are presented as the mean ± SD. N=3. *P<0.05, **P<0.01, ***P<0.001 vs. LPS control. LPS, lipopolysaccharide; TLR4, Toll-like receptor 4; L, LPS; A, human anti-TLR4 IgG2; IL, interleukin; IFN-β, interferon-β; TNF-α, tumor necrosis factor-α.