Table II.
Particle size and ζ-potential of DOTAP-based liposomes and lipoplexes of siRNA.
| Liposomes | Lipoplexesb | |||||
|---|---|---|---|---|---|---|
| Liposome | Sizea (nm) | PDI | ζ-potentiala (mV) | Sizea (nm) | PDI | ζ-potentiala (mV) |
| LP-DOTAP | 105.0±0.5 | 0.19±0.01 | 44.0±6.1 | 160.9±0.7 | 0.18±0.01 | 38.1±0.8 |
| LP-DOTAP-PEG-DSPE (1 mol%) | 108.8±0.7 | 0.24±0.01 | 46.5±7.9 | 179.4±1.5 | 0.24±0.02 | 40.5±1.6 |
| LP-DOTAP-PEG-DSPE (2 mol%) | 88.7±2.0 | 0.26±0.01 | 40.5±0.6 | 158.1±2.9 | 0.24±0.01 | 41.2±0.6 |
| LP-DOTAP-PEG-DSPE (3 mol%) | 114.5±2.3 | 0.28±0.02 | 37.9±0.4 | 174.3±2.7 | 0.27±0.00 | 37.1±1.3 |
| LP-DOTAP-PEG-DSG (1 mol%) | 113.7±0.4 | 0.25±0.01 | 49.7±1.2 | 171.1±3.3 | 0.23±0.01 | 32.2±1.4 |
| LP-DOTAP-PEG-DSG (2 mol%) | 118.6±1.6 | 0.26±0.00 | 39.3±0.5 | 174.2±3.0 | 0.26±0.01 | 40.2±0.5 |
| LP-DOTAP-PEG-DSG (3 mol%) | 117.7±1.2 | 0.25±0.01 | 40.4±0.6 | 155.7±2.8 | 0.26±0.01 | 40.2±0.4 |
| LP-DOTAP-PEG-Chol (1 mol%) | 111.2±12.9 | 0.16±0.13 | 37.3±2.2 | 181.8±1.3 | 0.23±0.02 | 35.9±2.1 |
| LP-DOTAP-PEG-Chol (2 mol%) | 112.3±4.5 | 0.26±0.02 | 41.2±1.7 | 170.4±0.4 | 0.20±0.01 | 39.4±0.7 |
| LP-DOTAP-PEG-Chol (3 mol%) | 92.8±0.5 | 0.21±0.01 | 40.1±3.9 | 162.0±3.8 | 0.26±0.04 | 34.2±1.9 |
a
In water.
b
Charge ratio (+:-) of cationic lipid to siRNA phosphate=4:1. Data are presented as the mean ± SD (n=3). DOTAP, 1,2-Dioleoyl-3-trimethylammonium-propane methyl sulfate salt; siRNA, small interfering RNA; LP, liposome; PEG, polyethylene glycol; PDI, polydispersity index.