Table IV.
Particle size and ζ-potential of PEG-CS coated siRNA lipoplexes.
| Lipoplex | Charge ratioa (+:-:-) | Sizeb (nm) | PDI | ζ-potentialb (mV) |
|---|---|---|---|---|
| LP-DOTAP-PEG-CS (1 mol%) | 4:1:0.9 | 250.0±1.7 | 0.20±0.01 | 39.7±0.1 |
| LP-DOTAP-PEG-CS (2 mol%) | 4:1:1.8 | 192.5±2.3 | 0.15±0.03 | −26.0±0.5 |
| LP-DOTAP-PEG-CS (3 mol%) | 4:1:2.7 | 183.6±2.1 | 0.12±0.00 | −32.4±1.1 |
| LP-DDAB-PEG-CS (1 mol%) | 4:1:0.9 | 211.0±0.9 | 0.11±0.01 | 41.6±0.5 |
| LP-DDAB-PEG-CS (2 mol%) | 4:1:1.8 | 165.8±0.6 | 0.12±0.01 | −28.9±1.2 |
| LP-DDAB-PEG-CS (3 mol%) | 4:1:2.7 | 166.8±1.7 | 0.12±0.01 | −39.2±1.0 |
| LP-TC-1-12-PEG-CS (1 mol%) | 4:1:0.9 | 168.6±1.5 | 0.13±0.02 | 27.0±1.8 |
| LP-TC-1-12-PEG-CS (2 mol%) | 4:1:1.8 | 212.1±41.1 | 0.10±0.02 | −28.3±1.9 |
| LP-TC-1-12-PEG-CS (3 mol%) | 4:1:2.7 | 161.6±1.5 | 0.10±0.02 | −26.9±1.1 |
a
Charge ratio (+:-:-) was calculated as a molar ratio of cationic lipid:siRNA phosphate:sulfate and carboxylic acid of CS.
b
In water. Data are presented as the mean ± SD (n=3). PEG, polyethylene glycol; CS, chondroitin sulfate C sodium salt; siRNA, small interfering RNA; LP, liposome; PDI, polydispersity index.