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. 2020 Sep 2;22(5):3677–3686. doi: 10.3892/mmr.2020.11490

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Copyright: © Xu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Functional role of SNHG12 in RCC cell lines. (A) Transfection efficiency of si-SNHG12 in A498 and 786O cell lines was analyzed using reverse transcription-quantitative PCR. (B) Cell viability was analyzed using an MTT assay following SNHG12 knockdown. (C) Cell invasive ability was detected using a Transwell assay following SNHG12 knockdown. Magnification, ×100; scale bar, 100 µm. (D) Flow cytometric analysis of apoptosis was performed following SNHG12 knockdown. Untransfected RCC cells were used as the ‘blank’ control. *P<0.05. SNHG12, small nucleolar RNA host gene 12; RCC, renal cell carcinoma; si, small interfering RNA; NC, negative control; 7-AAD, 7-Aminoactinomycin D.