Table 2.
Mutation frequency per ethyl methanesulphonate (EMS) treatment.
| Sample | EMS level (M) | SNV count | Minimum no. of mutants required to identify causal mutations | ||||
|---|---|---|---|---|---|---|---|
| GATK | Samtools mpileup | High confidence SNV | High + low confidence | Based on high confidence SNVs | Based on low confidence SNVs | ||
| GATK + Samtools shared | Nonredundant SNVs | ||||||
| B1 | 0.015 | 1,490 | 1,893 | 351 | 3,032 | 3 | 5 |
| B2 | 0.015 | 1,678 | 2,358 | 524 | 3,512 | 3 | 5 |
| C2 | 0.025 | 1,680 | 1,920 | 433 | 3,167 | 3 | 5 |
| D1 | 0.05 | 1,861 | 2,307 | 701 | 3,467 | 3 | 5 |
| D2 | 0.05 | 1,894 | 1,889 | 719 | 3,064 | 3 | 5 |
| E1 | 0.075 | 2,289 | 2,599 | 1,033 | 3,855 | 4 | 5 |
| E3 | 0.075 | 1,829 | 1,939 | 855 | 2,913 | 3 | 5 |