TABLE 2.
PCR primers used in this study.
| Primers | Sequences (5′-3′)a | Aim |
| MotA-UP-KpnI-F | GGTACCTCGCGGGTGTAGGCGACG | Construction of motA mutant |
| MotA-UP-NdeI-R | CATATGGCCCATGGCCATGAAGCC | Construction of motA mutant |
| MotA-DOWN-AgeI-F | ACCGGTAAGACCATTGCGGAAGGC | Construction of motA mutant |
| MotA-DOWN-SacI-R | GAGCTCACCGCGAAGTCCATGCGGGT | Construction of motA mutant |
| 2281-UP-KpnI -F | GGGGTACCACATCGTCATCCATTCCG | Construction of azc_2281 mutant |
| 2281-UP-NdeI-R | GGAATTCCATATGCAACTGATGGATACCCG | Construction of azc_2281 mutant |
| 2281-DOWN-AgeI-F | GACCGGTATTCATTTTACGCATGATG | Construction of azc_2281 mutant |
| 2281-DOWN-SacI-R | CGAGCTCTCATCAGCGCCAGCCAGT | Construction of azc_2281 mutant |
| 3095-UP-KpnI-F | GGGGTACCTGAACGTCTGGTGGAATG | Construction of azc_3095 mutant |
| 3095-UP-NdeI-R | GGAATTCCATATGATGGCTGCGGTGCGCTAT | Construction of azc_3095 mutant |
| 3095-DOWN-AgeI-F | GACCGGTCACTGGCCCTGTCGGAA | Construction of azc_3095 mutant |
| 3095-DOWN-SacI-R | CGAGCTCCAACCATATGAGCTTCCT | Construction of azc_3095 mutant |
| 4108-UP-KpnI-F | GGGGTACCCGCCTGCTGCTCTCGGAA | Construction of azc_4108 mutant |
| 4108-UP-NdeI-R | GGAATTCCATATGTGCGCAGAGCCTCCAGAA | Construction of azc_4108 mutant |
| 4108-DOWN-AgeI-F | GACCGGTGCCCTGGCGAGCGTCTAG | Construction of azc_4108 mutant |
| 4108-DOWN-SacI-R | CGAGCTCGAACCTAACGCTCGACTC | Construction of azc_4108 mutant |
aengineered restriction sites are underlined.