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. 2020 Sep 18;11:576547. doi: 10.3389/fphar.2020.576547

Figure 1.

Figure 1

Ziyuglycoside II inhibits colorectal cancer cells proliferation both in vitro and in vivo. (A). Chemical structure of Ziyu II. MTT assay of colorectal cancer lines incubated with the designated concentrations of Ziyu II for 24 h (B). EdU incorporation (C), LDH release assay in cells incubated as in (F). Colony formation assay of DLD-1 and HCT116 cells intervented with the designated concentrations of Ziyu II for 2 weeks. Characteristic images (D) and quantification of colonies (E) were shown. G–J, NOD-SCID mice were injected with DLD-1 cells and intervented with Ziyu II or vehicle. Tumor volumes were measured at designated time points (I). Photograph of dissected tumors derived from control or Ziyu II-treated mice (G). Tumor weights at time of sacrifice (H).The expression of Ki67 was detected by IHC in tumor xenografts (J), and relative immunohistochemical scores were shown (K) Scale bar, 50 μm. All data are means ± SD. **p < 0.01; ***p < 0.001.