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. 2020 Sep 18;11:576547. doi: 10.3389/fphar.2020.576547

Figure 2.

Figure 2

Ziyuglycoside II induces apoptosis in colorectal cancer cells in vitro. DLD-1 and HCT116 cells were subjected to Ziyu II Dox 10uM for 24 h, and flow cytometric analysis of apoptosis (A). (B) MTT assay of DLD-1 and HCT116 cells indicated with the presence or absence of Ziyu II, and in combination with or without ZVAD for 24 h. Colony formation assay of DLD-1 and HCT116 cells indicated with the presence or absence of Ziyu II, and in combination with or without ZVAD for 2 weeks. The characteristic images (C) and count of colonies (D) were shown. (E) Flow cytometric analysis of ROS in DLD-1 and HCT116 cells treated with the indicated concentration of Ziyu II for 24 h. (F). MTT assay of DLD-1 and HCT116 cells indicated with NAC for 24 h. All data are means ± SD. *p < 0.05; **p < 0.01; ***p < 0.001.