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. 2020 Sep 18;11:576547. doi: 10.3389/fphar.2020.576547

Figure 3.

Figure 3

Ziyuglycoside II induces autophagy in colorectal cancer cells both in vitro and in vivo. (A) The protein of LC3, Atg7, and ATG5 of DLD-1 and HCT116 cells were detected by Western blot after 24 h of treatment with Ziyu II. (B) Immunoblotting of LC3 in DLD-1 and HCT116 cells indicated the presence or absence of Ziyu II, and in combination with or without 3 MA for 24 h (C) Formation of endogenous LC3 puncta in cells exposed to DMSO or Ziyu II for 24 h and (E) total number of endogenous LC3 puncta per cell. (D), the formation of exogenous GFP-LC3 puncta in cells treated with DMSO or Ziyu II for 24 h and (F) the total number of exogenous LC3 puncta per cell. Scale bars: 10 μm. LC3 expression in orthotopic xenografts was examined by IHC. Representative images were provided as indicated (G), and relative immunohistochemical scores were shown (H). Scale bar, 50 μm. All data are means ± SD. **p < 0.001.