Table 1.
Top 60 low abundant proteins identified in whey proteome with respective database information, molecular function, cellular component, biological process, Prosite, Pfam, InterPro, SMART, and SUPFAM.
| Protein | Protein names | Gene symbol | ng | Molecular function | Cellular component | Biological process | Prosite | Pfam | InterPro | SMART | SUPFAM |
|---|---|---|---|---|---|---|---|---|---|---|---|
| F1MCK2 | AHNAK nucleoprotein | AHNAK | 0.074 | Costamere | Regulation of RNA splicing | PS50106 | IPR001478 | SM00228 | SSF50156 | ||
| E1BPD7 | Marker of proliferation Ki-67 | MKI67 | 0.066 | PS50006 | PF00498 | IPR000253 | SM00240 | SSF49879 | |||
| F1N415 | Piccolo presynaptic cytomatrix protein | PCLO | 0.075 | Metal ion binding | Presynaptic active zone | Synapse assembly | PS50004 | PF00168 | IPR000008 | SM00239 | SSF50156 |
| E1BGB0 | Kinesin family member 13B | KIF13B | 0.054 | 14-3-3 protein binding | Axon | Microtubule-based movement | PS00845 | PF01302 | IPR036859 | SM01052 | SSF49879 |
| F1MV51 | APC regulator of WNT signaling pathway | APC | 0.094 | Beta-catenin binding | Beta-catenin complex | Cell cycle arrest | PS50176 | PF05972 | IPR026836 | SM00185 | SSF48371 |
| E1BLA0 | Shugoshin 2 | SGOL2 | 0.057 | Centromeric region | Meiotic sister chromatid cohesion | IPR026706 | |||||
| E1BKT9 | Desmoplakin | DSP | 0.018 | Cell adhesion molecule binding | Plasma membrane | Adherens junction organization | PS50002 | PF00681 | IPR028462 | SM00250 | SSF75399 |
| E1BPB1 | Kinesin family member 24 | KIF24 | 0.093 | ATPase activity | kinesin complex | Microtubule-based movement | PS00411 | PF00225 | IPR027640 | SM00129 | SSF47769 |
| L8IEV2 | Dynein heavy chain 1, axonemal | M91_21125 | 0.078 | ATPase activity | Dynein complex | Cilium movement | PF12774 | IPR035699 | SSF52540 | ||
| E1BHP0 | Alpha-mannosidase | MAN2B2 | 0.095 | Alpha-mannosidase activity | Vacuolar membrane | Mannose metabolic process | PF09261 | IPR011013 | SM00872 | SSF74650 | |
| L8IV13 | Leucine-rich repeat-containing protein KIAA1731 | M91_18003 | 0.083 | Microtubule binding | Centriole | Positive regulation of centriole elongation | PF15309 | IPR029299 | |||
| L8HSL9 | WD repeat-containing protein 87 | M91_17430 | 0.085 | PS50082 | PF00400 | IPR015943 | SM00320 | SSF50978 | |||
| E1BKZ5 | Golgin B1 | GOLGB1 | 0.084 | cis-Golgi network | Protein localization to pericentriolar material | IPR026202 | |||||
| F1N2K7 | Phosphoinositide kinase, FYVE-type zinc finger containing | PIKFYVE | 0.033 | 1-Phosphatidylinositol-3-phosphate 5-kinase activity | Cell–cell junction | Intracellular signal transduction | PS50186 | PF00118 | IPR002423 | SM00049 | |
| E1BC24 | Midasin | MDN1 | 0.062 | ATPase activity | Cytosol | Ribosomal large subunit assembly | PS50234 | PF07728 | IPR003593 | SM00382 | SSF52540 |
| E1BKC4 | Nipped-B protein | NIPBL | 0.071 | Chromatin binding | Chromatin | Brain development | PF12765 | IPR011989 | SSF48371 | ||
| L8IT59 | Vacuolar protein sorting-associated protein 13A | M91_05527 | 0.081 | PF09333 | IPR015412 | ||||||
| E1BKI3 | Nitric oxide synthase (EC 1.14.13.39) | NOS1 | 0.065 | Calmodulin binding | Cytosol | Arginine catabolic process | PS51384 | PF00667 | IPR003097 | SM00228 | SSF50156 |
| E1B9N6 | Envoplakin | EVPL | 0.035 | Intermediate filament binding | Cornified envelope | Epidermis development | PF00681 | IPR041615 | SM00250 | SSF75399 | |
| E1BPX1 | Vacuolar protein sorting 13 homolog C | VPS13C | 0.103 | Cytosol | Mitochondrion organization | PF09333 | IPR015412 | ||||
| O46382 | Brefeldin A-inhibited guanine nucleotide-exchange protein 1 | ARFGEF1 | 0.091 | ARF guanyl-nucleotide exchange factor activity | Cytosol | Endomembrane system organization | PS50190 | PF16213 | IPR016024 | SM00222 | SSF48371 |
| F1MC51 | ATP binding cassette subfamily B member 5 | ABCB5 | 0.067 | ATPase activity | Plasma membrane | PS50929 | PF00664 | IPR003593 | SM00382 | SSF52540 | |
| E1BHT5 | Ubiquitin protein ligase E3 component n-recognin 4 | UBR4 | 0.065 | Ubiquitin-protein transferase activity | Centrosome | Ubiquitin-dependent protein catabolic process | PS51157 | PF13764 | IPR016024 | SM00396 | SSF48371 |
| E1BCI2 | E3 ubiquitin protein ligase (EC 2.3.2.27) | RNF40 | 0.061 | Metal ion binding | HULC complex | Histone H2B ubiquitination | PS00518 | IPR013956 | SM00184 | ||
| E1BL95 | Centromere protein J | CENPJ | 0.037 | Identical protein binding | Centriole | Microtubule polymerization | PF07202 | IPR033068 | |||
| E1B7W1 | Thyroid hormone receptor associated protein 3 | THRAP3 | 0.092 | RNA splicing | PF15440 | IPR026667 | |||||
| F1N137 | Inositol 1,4,5-trisphosphate receptor type 2 | ITPR2 | 0.094 | Inositol 1,4,5 trisphosphate binding | Endoplasmic reticulum | PS50919 | PF08709 | IPR014821 | SM00472 | SSF100909 | |
| F6RF21 | Structural maintenance of chromosomes flexible hinge domain containing 1 | SMCHD1 | 0.047 | ATPase activity | Barr body | Double-strand break repair | PF06470 | IPR036890 | SM00968 | SSF55874 | |
| F1MHT1 | Amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase | AGL | 0.047 | 4-Alpha-glucanotransferase activity | Cytoplasm | Glycogen biosynthetic process | PF06202 | IPR008928 | SSF48208 | ||
| L8J1D8 | Eukaryotic translation initiation factor 3 subunit B (eIF3b) | EIF3B | 0.072 | Translation initiation factor activity | 43S complex | Cytoplasmic translation initiation complex | PS50102 | PF08662 | IPR011400 | SM00360 | SSF54928 |
| F1MJ95 | Unc-80 homolog, NALCN channel complex subunit | UNC80 | 0.081 | Cation channel activity | Axon | Cation homeostasis | PF15778 | IPR031542 | |||
| E1B9N8 | Lysine methyltransferase 2D | KMT2D | 0.033 | Histone binding | MLL3/4 complex | RNA polymerase II | PS51805 | PF05965 | IPR034732 | SM00542 | SSF47095 |
| F1MWK8 | Protein tyrosine kinase 7 (inactive) | PTK7 | 0.089 | ATP binding | Cell–cell junction | Actin cytoskeleton reorganization | PS50835 | PF07679 | IPR007110 | SM00409 | SSF48726 |
| E1BCV4 | Nucleoporin 98 | NUP98 | 0.059 | mRNA binding | Kinetochore | Nuclear pore complex assembly | PS51434 | PF04096 | IPR037665 | SSF82215 | |
| A4IFK4 | Synaptopodin 2 | SYNPO2 | 0.076 | Actin binding | Cytoskeleton | Actin bundle assembly | PS50106 | PF00595 | IPR001478 | SM00228 | SSF50156 |
| A6QLI5 | Melanoma-associated antigen D4 (MAGE-D4 antigen) | MAGED4 | 0.094 | PS50838 | PF01454 | IPR037445 | SM01373 | ||||
| E1BLB6 | Protein kinase, DNA-activated, catalytic subunit | PRKDC | 0.086 | ATP binding | DNA-dependent kinase- ligase 4 complex | Activation of innate immune response | PS51189 | PF02259 | IPR016024 | SM01343 | SSF48371 |
| F1MGR3 | Arginine-glutamic acid dipeptide repeats | RERE | 0.100 | Chromatin binding | Histone deacetylase | Branching of a nerve | PS51038 | PF03154 | IPR002951 | SM00439 | SSF46689 |
| A6H737 | Lysyl oxidase homolog 2 (Lysyl oxidase-like protein 2) | LOXL2 | 0.080 | Calcium ion binding | Basement membrane | Cellular protein modification process | PS00926 | PF01186 | IPR001695 | SM00202 | SSF56487 |
| A6QQJ9 | GPRASP1 protein | GPRASP1 | 0.085 | PF04826 | IPR006911 | SSF48371 | |||||
| F1MZM7 | Synapse defective Rho GTPase homolog 2 | SYDE2 | 0.054 | Signal transduction | PS50238 | PF00620 | IPR035892 | SM00324 | SSF48350 | ||
| A0JN33 | Kin of IRRE like 3 | KIRREL3 | 0.083 | Plasma membrane | PS50835 | PF07679 | IPR007110 | SM00409 | SSF48726 | ||
| F1MNU5 | Phospholipase A2 | PLA2G4F | 0.078 | Calcium-dependent phospholipase A2 | Cytoplasm | Arachidonic acid secretion | PS50004 | PF00168 | IPR016035 | SM00239 | SSF52151 |
| E1B949 | FAT atypical cadherin 4 | FAT4 | 0.086 | Calcium ion binding | Apical part of cell | Branching involved in ureteric bud | PS00010 | PF00028 | IPR002126 | SM00112 | |
| F1MZU6 | Collagen alpha-3(IV) chain | COL4A3 | 0.048 | Extracellular matrix | Collagen | Apoptotic process | PS51403 | PF01413 | IPR008160 | SM00111 | SSF56436 |
| E1BEB3 | Histone acetyltransferase | KAT6A | 0.102 | DNA binding | Cytosol | Cellular senescence | PS51504 | PF01853 | IPR016181 | SM00526 | SSF46785 |
| E1BKZ9 | Sortilin | SORT1 | 0.070 | Cytosol | Endocytosis | PF15902 | IPR031777 | SM00602 | |||
| L8HZQ1 | Kielin/chordin-like protein | M91_00882 | 0.063 | PS01208 | PF00093 | IPR036084 | SM00832 | SSF57567 | |||
| F1MG90 | WDFY family member 4 | WDFY4 | 0.023 | PS50197 | PF02138 | IPR016024 | SM01026 | ||||
| L8J3H5 | Sperm-associated antigen 1 | M91_19629 | 0.096 | PS50005 | PF13877 | IPR025986 | SM00028 | SSF48452 | |||
| E1BHN4 | RING-type domain | RNF213 | 0.063 | ATPase activity | Cytoplasm | Wnt signaling pathway | PS00518 | PF00097 | IPR003593 | SM00382 | SSF52540 |
| F1MFU7 | Zinc finger protein 862 | ZNF862 | 0.069 | Nucleic acid binding | Transcription | PS50805 | PF05699 | IPR008906 | SM00349 | SSF10964 | |
| E1BNA3 | Adaptor related protein complex 4 subunit epsilon 1 | AP4E1 | 0.064 | AP-4 adaptor complex | Vesicle-mediated transport | PF01602 | IPR017109 | SM01356 | SSF48371 | ||
| P98167 | SCO-spondin | SSPO | 0.044 | Peptidase inhibitor | Extracellular | Cell adhesion | PS01225 | PF08742 | IPR006207 | SM00832 | KOG1215 |
| E1BEJ9 | NLR family apoptosis inhibitor | NAIP | 0.056 | ATP binding | Plasma membrane | Apoptotic process | PS50143 | PF00653 | IPR003593 | SM00382 | SSF52540 |
| F1MFH3 | FRAS1 related extracellular matrix protein 2 | FREM2 | 0.055 | Basement membrane | Cell communication | PS51854 | PF03160 | IPR038081 | SM00237 | SSF141072 | |
| E1B754 | Microtubule associated serine/threonine kinase 2 | MAST2 | 0.086 | ATP binding | Microtubule cytoskeleton | Cytoskeleton organization | PS51285 | PF08926 | IPR000961 | SM00228 | |
| E1BP05 | Usherin | USH2A | 0.092 | Collagen binding | Apical plasma membrane | Animal organ morphogenesis | PS01248 | PF00041 | IPR013320 | SM00180 | SSF49265 |
| A3KN18 | CEBPZ protein | CEBPZ | 0.067 | Ribosome biogenesis | PF03914 | IPR016024 | SSF48371 |