Fig. 4.

Egg-to-pupa survival rate. Females and males heterozygous for the drive and Cas9 alleles were crossed with w¹¹¹⁸ flies, and w¹¹¹⁸ individuals were crossed together. Females were allowed to lay eggs for 1 d, which were counted and then assessed for survival. Each data point represents the survival rate of eggs to the pupa stage observed in one vial containing one individual. The size of each dot represents the total number of eggs laid in the vial. The progeny of heterozygous females had a significantly lower survival rate than wild-type flies but the progeny of heterozygous males did not, with the difference presumably caused by reduced viability from nonfunctional resistance alleles that formed in the embryo due to maternally deposited Cas9 and gRNAs. An alternate analysis that accounts for potential batch effects was also performed but yielded overall similar rates with only slightly increased error estimates (Dataset S1 A–C).