Fig. 2.

NR4A3 restrains cytokine production by CD8⁺ T cells. (A) Day 7 postinfection with Lm-OVA, OT-I effectors were restimulated for 5 h with OVA peptide and assessed for cytokine secretion. The percentage of positive OT-I cells for each cytokine and mean fluorescence intensity (MFI) on cells positive for the measured cytokines are shown. (B) Retrovirally transduced GFP⁺ cells, as in Fig. 1C, were sorted prior to restimulation in order to measure cytokine production. ^+/+: Nr4a3^+/+ cells; ^−/−: Nr4a3^−/− cells. Data are from two (B) or at least three independent experiments. Unpaired Student’s t test, with a Welch’s correction when applied, was used for two-group comparison and Kruskal–Wallis ANOVA with Dunn’s multiple comparison for multiple group comparison: *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.