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. 2020 Sep 14;117(39):24503–24513. doi: 10.1073/pnas.2004087117

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Fig. 1. — UNC5B interacts with MST1. (A) GST pulldown with HA-UNC5H2 and GST-MST1 cotransfected HEK293 cell lysates. Immunoblots show that MST1 interacted with UNC5B in cotransfected HEK293 cells. (B) Mst1 selectively interacts with UNC5H2. GST pulldown from HA-UNC5H1, UNC5BH2, and UNC5H3 and GST-MST1 cotransfected HEK293 cell lysates was detected by immunoblotting. (C) Mst2 does not bind to any UNC5 receptors. IP of Flag tag from HA-UNC5H1, UNC5BH2, and UNC5H3 and Flag-MST1 cotransfected HEK293 cell lysates was analyzed by immunoblotting. (D) Endogenous MST1 interacts with UNC5B in the brain. CO-IP of MST1 and UNC5B proteins in the control or Cre virus-injected UNC5B f/f mice brain lysates was analyzed by immunoblotting. (E) IP of UNC5B from the brain lysates of MST1 f/f mice injected with Cre or control virus was confirmed by immunoblotting. Immunoblots show that MST1 interacted with UNC5B. Three independent studies were conducted in all of the experiments.