Fig. 1.
Exacerbated disease in mice depleted of microglia. Mice were infected intracranially with 700-PFU JHMV, then fed at day 7 p.i. with PLX5622-containing or control chow (A–F). Representative flow plots of spinal cords showing gating for lymphocytes (CD45+CD11b−), monocytes/macrophages (CD45hiCD11b+, MΦ), and microglia (CD45intCD11b+) at day 14 p.i. (B). Summary of numbers of microglia in spinal cords at day 14 p.i. (C). Clinical scores at indicated days p.i. (D). (B–D n = 10 mice/group). Expression levels of viral genomic RNA (gRNA) as assessed by qPCR (E) (n = 4–13 mice/group) and infectious virus titers as determined by plaque assay in the spinal cord (F) (n = 4–9 mice/group). Mice were infected and fed at day 10 (G and H) or day 15 (I and J) p.i. with PLX5622-containing or control chow. Mice were infected and fed PLX5622-containing or control chow at day 7, followed by replacement of PLX5622-containing chow with control chow at 14 dpi (K and L). Clinical scores at indicated days p.i. (H, J, and L) (n = 5–10 mice/group). Data represent the mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 by Mann–Whitney U test.