Fig. 4.

Microglia express proremyelination and myelin debris removal genes. Microglia from mice at 14 and 21 dpi as well as mock-infected mice were isolated by a fluorescence-activated cell sorter for RNA-seq analysis. Heat maps show the 1,818 unique differentially expressed genes across each pairwise comparison (A) and selected genes involved in debris clearance and remyelination (B). Isolated microglia at 14 dpi and after mock infection and isolated monocytes/macrophages (MΦ) at 14 dpi from control- and PLX5622-treated mice were analyzed for the indicated mRNA transcripts by qPCR (C). Following infection and treatment with PLX5622-containing or control feed at 7 dpi, flow cytometry was used to analyze the geometric gMFI of CD11c in monocytes/macrophages at 14 dpi (D). Data in A and B were derived from four mice per group and analyzed as described in Materials and Methods. Data in C represent 4–9 mice per group. Data in D represent combined results from two to three experiments with a combined total of 5–17 mice per group. n.d.: undetermined Ct values. Data represent the mean ± SEM, *P < 0.05, ***P < 0.001, ****P < 0.0001 by Mann–Whitney U test.