. 2020 Sep 10;117(39):24459–24463. doi: 10.1073/pnas.2011299117

Table 1.

Kinetic parameters of Methylacidiphilum fumariolicum SolV HAO (mHAO) compared to its characterized homologs from Kuenenia stuttgartiensis (KsHAO) and Nitrosomonas europaea (NeHAO)

Enzyme	mHAO^*	KsHAO^†	NeHAO^‡
V_max, µmol NH₂OH∙min⁻¹∙mg HAO⁻¹	5.5	4.8	9.5
K_m, µM	1.4	4.4	3.6
K_cat, s⁻¹	7.2	4.9	10.5
K_cat/K_m, s⁻¹∙µM⁻¹	5.1	1.1	2.9

pH 7.5, 60 °C.

^{^†}

pH 7.0, 37 °C; values calculated from data presented by Maalcke et al. (27).

^{^‡}

pH 9.5, room temperature; values calculated from data presented by Hooper and Nason (22).