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Degenerate 16S rRNA primers for PCR assays.
| 16S rDNA regions | Degenerate primer sequence (5′-3′) | Tm (oC) | Amplicon length (bases) | |
|---|---|---|---|---|
| (V1V2) | F: | TWACACATGCAAGTCGARCG | 56–59 | 339 |
| R: | CAAWATTCCCCACTGCTGCC | 58–59 | ||
| (V4V5) | F: | CAGCCGCGGTAATACGDAGG | 60–63 | 380 |
| R: | TGCGRCCGTACTCCCCAGGC | 60–63 | ||
| (V6V7V8) | F: | CGA WGCAACGCGAARAACCT | 60–61 | 362 |
| R: | CGAGTTGCAGACTVCAATCCG | 59–62 | ||
Degenerate bases (W = A/T, R = A/G, and D = A/G/T)
