Fig. 2.

Effects of oleic acid on C2C12 myotube treated with SCD inhibitor. C2C12 cells were starved for 6 days to be differentiated into myotubes cells. Myotube cells were treated with vehicle (Veh), 1 µM SCD inhibitor (SCDi, CAY10566), and 300 µM oleic acid (18:1) for 48 h (A–C) or 12 h (D). (A) Representative images of myotubes treated as indicated. Scale bar = 100 µm. (B) The measurement of diameters as described in MATERIAL and METHODS. Mean ± SEM. (n = 100). (C, D) The muscle-specific ubiquitin E3 ligases (Atrogin1 and MuRF1) and the UPR (BiP, sXBP-1, ATF4, and CHOP) mRNA expression were evaluated by real-time PCR analysis. Mean ± SEM. (n = 3). *p<0.05 vs Veh, ^#p<0.05 vs SCDi.