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. 2020 Mar 6;67(2):137–145. doi: 10.3164/jcbn.19-121

Fig. 1.

Fig. 1

Quercetin induces autophagic cell death in MG63 cells. (A) MG63 cells were treated with different concentrations of quercetin for 24 h, and the expression levels of the autophagy-associated proteins LC3B-I/II, and p62/SQSTM1 were then assessed by western blotting. MG63 cells were pretreated with 100 nM Baf.A1 for 2 h and incubated with 200 µM quercetin for another 24 h. (B) The LC3B-II levels were then assessed by western blotting; MG63 cells were preincubated with (C, D) 100 nM Baf.A1 for 2 h or ATG5-shRNA for 24 h and then treated with 200 µM quercetin for another 24 h, then Trypan blue assay used to assess cell death. *p<0.05, **p<0.01 vs the control group, #p<0.05, ##p<0.01 vs the quercetin (200 µM) group (n = 4).