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NUPR1 mediates quercetin-induced autophagy. (A) The mRNA levels of NUPR1 were determined in NUPR1 shRNA-transfected MG63 cells using RT-PCR. (B, C) MG63 cells were treated with shRNA against NUPR1 or control shRNA and then incubated with 200 µM quercetin for another 24 h before measuring the Luciferase activity and NUPR1-target genes. (D) Trypan blue assay used to assess cell death. *p<0.01 vs the control group, #p<0.01 vs the Control-sh+quercetin (200 µM) group (n = 4).
