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NAC abolishes quercetin-induced NUPR1-depended autophagic cell death in vivo. Nude mice with human MG63 cells subcutaneous tumor xenografts were established and treated with vehicle control, 100 mg/kg quercetin, with or without 150 mg/kg NAC. (A, B) The mRNA and protein levels of NUPR1 were detected. (C) The mRNA levels of NUPR1-target genes were measured using RT-PCR. *p<0.01 vs the control group; #p<0.01 vs the quercetin group (n = 8).
