Figure 2.

CD8⁺ T cell subsets along the colonic tract. [A] t-SNE analysis of CD8⁺ cellular events isolated from intestinal biopsies derived from the caecum, transverse, or descending colon, or rectum. Density plots are displayed, alongside plots displaying CD103 and CD161 expression, generated using the OMIQ opt-SNE algorithm. [B] Proportions of the indicated CD8⁺ cellular subsets within each colonic compartment, determined using the Astrolabe profiling step,³² by limiting clusters to CD8⁺ events. [C] A heatmap displaying the relative expression of the cellular markers indicated, within the CD8⁺ T cell population, across the four colonic compartments. [D] Proportions of the indicated CD8⁺ T cell subsets within each colonic segment. Each data point represents a single donor, n = 10 total. Error bars represent standard deviation of mean. Statistical significance determined using analysis of variance [ANOVA], followed by Tukey’s multiple comparison test.