Figure 3.

CD4⁺ T cell subsets along the colonic tract. [A] opt-SNE analysis of CD4⁺ cellular events isolated from intestinal biopsies derived from the caecum, transverse, or descending colon, or rectum. [B] A heatmap displaying the relative expression of the cellular markers indicated, within the CD4⁺ T cell population, across the four colonic compartments. [C] Representative opt-SNE plots displaying expression of CD103, CD161, CCR6, and PD-1 among CD4⁺ cellular events. opt-SNE plots derived from the transverse colon from a single representative donor. [D] Summarised data of the expression of each marker in [C] displayed, determined using the Astrolabe profiling step,³² by limiting clusters to CD4⁺ events. Each data point represents a single donor, n = 10 total. Error bars represent standard deviation of mean. Statistical significance determined using analysis of variance [ANOVA], followed by Tukey’s multiple comparison test.