Figure 4.

B cell subsets along the colonic tract. [A] Representative opt-SNE analysis of CD19⁺/Lin^-CD38⁺ cellular events isolated from intestinal biopsies, derived from the caecum, transverse, or descending colon, or rectum. Density plots are displayed, alongside expression plots displaying the expression of CD19 and CD38. [B] Proportions of the indicated B cell lineage subsets within each colonic compartment, determined using the Astrolabe profiling step,³² by limiting clusters to CD19⁺/Lin^-CD38⁺ events. [C] Summary data displaying abundances of CD19⁺HLA-DR⁺ and CD19⁺ HLA-DR^- subsets within each colonic segment. [D] Summary data displaying abundances of CD38⁺CD27⁺ and CD38⁺CD27^- subsets within each colonic segment. Each data point represents a single donor, n = 10 total. Error bars represent standard deviation of mean. Statistical significance determined using analysis of variance [ANOVA], followed by Tukey’s multiple comparison test.