Figure 6.

NK and NKT cells along the colonic tract. [A] Representative t-SNE plot displaying events grouped into one of three sub-populations: CD3⁺CD161⁺, CD3^-CD161⁺, and CD3^-CD161^-, pre-gated on Lin^-CD56⁺ cellular events. In all, 25 000 cellular events were used for generation of t-SNE plots. [B] Abundance of CD3⁺CD161⁺ NKT cells, [C] CD3^-CD161⁺ NK cells, and [D] CD3^-CD161^- NK cells in the four colonic compartments examined, determined using the Astrolabe profiling step,³² by limiting clusters to Lin^-CD56⁺ events. Each data point represents a single donor, n = 10 total. Error bars represent standard deviation of mean. Statistical significance determined using analysis of variance [ANOVA], followed by Tukey’s multiple comparison test.