Table 3.
Detection of Phosphorylated Alpha-Synuclein Without Colocalization Using PGP 9.5.
| PD N=29 | Control N=21 | |||||
|---|---|---|---|---|---|---|
| Thickness | 50 | 20 | 10 | 50 | 20 | 10 |
| Distal leg (% P-syn positive) | 86% | 69% | 62% | 19% | 24% | 38% |
| Distal thigh (% P-syn positive) | 76% | 72% | 65% | 24% | 29% | 33% |
| Proximal thigh (% P-syn positive) | 86% | 65% | 72% | 19% | 29% | 38% |
Abbreviations: PD, Parkinson’s disease; PGP 9.5, protein gene product 9.5.
The sensitivity of detecting P-syn with results from three biopsies was 100% in the 50-, 20-, and 10-µm-thick tissue sections using PGP 9.5. The calculated specificity of detecting P-syn with results from three biopsies was 66% with 50 µm sections, 60% with 20 µm sections, and 57% with 10 µm sections without colocalizing with PGP 9.5.