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. 2020 Sep 27;58(1):999–1005. doi: 10.1080/13880209.2020.1818791

Figure 1.

Figure 1.

Effect of 5-CQA on cytotoxicity effect. (A) Chemical structure of 5-CQA. (B) MTT assays for cell viability. The effect of 5-CQA (0–200 μM, 24 h treatment) on cell viability was assessed using MTT assays. (C) Time course of nuclear translocation of Nrf2 by 5-CQA in HepG2 cells. Western blot was performed using nuclear fractions of cells incubated in the presence of 100 μM 5-CQA for 0–6 h. (D) Effect of varying concentrations of 5-CQA on Nrf2 nuclear translocation in HepG2 cells (6 h). (E) Increases in Nrf2 transactivation by 5-CQA. NQO1-ARE luciferase activity was measured in lysates of HepG2 cells stably transfected with NQO1-ARE luciferase construct exposed to 10–100 μM 5-CQA for 12 h. Data represent the mean ± SE of three replicates, with statistically significant differences between each treatment group and the control defined by *p < 0.05 or **p < 0.01.