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. 2020 Sep 27;58(1):999–1005. doi: 10.1080/13880209.2020.1818791

Figure 2.

Figure 2.

Effect of 5-CQA on expression of Nrf2 target genes. (A) Time course of Nrf2 target gene expression by 5-CQA. HepG2 cells were treated with 100 μM 5-CQA for 0 to 12 h. Glutamate-cysteine ligase (GCL), hemeoxygenase 1 (HO-1), NAD(P)H: quinone oxidoreductase 1 (NQO1) and Sestrin2 (Sesn2) were immunoblotted from the lysates of cells. (B) Effect of 5-CQA on tert-butyl hydroperoxide (t-BHP)-induced ROS production. HepG2 cells were treated with 500 μM t-BHP and/or 0–100 μM 5-CQA. Cells were stained with 10 μM DCFH-DA for 30 min at 37 °C. (C) Measurement of intracellular GSH. GSH was analysed in lysates of cells treated with 500 μM t-BHP and/or 0–100 μM 5-CQA. Data represent the mean ± SE of four replicates, where statistically significant differences between each treatment group and the control were defined by **p < 0.01 for vehicle-treated control and #p < 0.05 or ##p < 0.01 for t-BHP alone.