Figure 5.

Specific role of Nrf2 on cytoprotective effect of 5-CQA. (A) Inhibition of 5-CQA-induced GCL and HO-1 expression by Nrf2 inhibitor. Cells were concomitantly treated with 100 μM 5-CQA or 5 μM ML-385. (B) Effect of 5-CQA on GCL and HO-1 induction in wild type (WT) or Nrf2-knockout (KO) MEF cells. Western blot assay was performed on lysates of WT or Nrf2-KO MEF cells treated with 5-CQA for 12 h. (C) The effect of 5-CQA (10–100 μM, 1 h pre-treatment) in the presence or absence of t-BHP (500 μM, 12 h) on cell viability was assessed using MTT assays in WT or Nrf2-KO MEF cells. Data represent the mean ± SE of four replicates; **p < 0.01, significant versus vehicle-treated control; ^##p < 0.01, significant versus t-BHP alone.