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Caspase-3 activation activity of compounds 4a–k. U937 5 × 105 cells/well seeding (2.5 × 105 cells/ml, 2 ml/well, six well) were treated with PAC-1 or compounds (50 µM) for 24 h. Cell lysate was used to detect caspase-3 activation by caspase-3 assay kit. UN: untreated; VH: vehicle (DMSO 0.05%).
