Figure 5.

Competing the neutralizing activity of convalescent serum with dominant B-cell linear epitopes. (a) Reaction of the neutralizing antibodies (Nab) and non-neutralizing antibodies (Non-Nab) with the peptide array. The peptides with signal to noise above 2.1 were counted as positive (black bar). (b) Schematic of experiment design. Four neutralizing sera (P13, P18, P68 and P98) were diluted and incubated with SARS-CoV-2 and the peptides (S1-55, S1-57, S2-13 and S2-47), respectively before added to the media. At 24 p.i, the viral RNAs in the supernatants were quantified by q-PCR. Experiment was done in duplicated well for each serum (b) Quantification of the viral RNA levels in the supernatants from representative wells. Relative RNA levels to the mock-treated wells were plotted (n = 2).