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. 2020 Sep 16;27(1):1319–1328. doi: 10.1080/10717544.2020.1815897

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© 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Nuclear (Hoechst 33258) staining assay for examining Free PT, Free REGO, PT NPs, REGO NPs, and REGO-PT NPs-induced cell death in A549 cells. The cells were treated with Free PT, Free REGO, PT NPs, REGO NPs, and REGO-PT NPs at 2.5 µM concentration for 24 h. B) Quantification of apoptosis ratio. The cells were quantified by image J software.