Figure 5.

Immunofluorescent staining to detect expression of PPAR signaling pathway genes in infected cells. (a) Protein-level expression of PPAR pathway genes was monitored by immunostaining with antibodies against FGR, APOC3 or HELZ2 (red) in ZIKV-infected and mock-infected ReNcell CX cells. Infected cells were identified by staining with antibody against ZIKV Envelope protein (green) and nuclei were stained using DAPI (blue). Scale bar is 100 µm. (b) Protein-level expression of PPAR pathway gene RXRG was monitored by immunostaining with antibodies against the protein (red) in ZIKV-infected and mock-infected ReNcell CX cells. Infected cells were identified by staining with antibody against ZIKV Envelope protein (green) and nuclei were stained using DAPI (blue). Scale bar is 100 µm. A 2X zoom of a specific region in the infected cell field of view is shown to indicate cytoplasmic staining of RXRG in infected cells. White arrows indicate cytoplasmic staining of the protein in infected cells and yellow arrows indicate RXRG staining limited to the nucleus in mock-infected cells.