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. Author manuscript; available in PMC: 2020 Oct 5.
Published in final edited form as: Biochemistry. 2019 Jun 28;58(28):3087–3096. doi: 10.1021/acs.biochem.9b00193

Table 5.

Cyt. c reduction rates of iNOS holoenzyme in the presence of crowder.a

[Crowder] (w/v) Cyt. c reduction (min−1)
0 % 1403 ± 49
3% Ficoll 70 1510 ± 38
7% Ficoll 70b 1668 ± 70
11% Ficoll 70 1795 ± 108
14% Ficoll 70c 1928 ± 130
4.6% Dextran 70b 1680 ± 88
7.5% Dextran 70c 1947 ± 122
a

Rates are the average of at least three assays. The final concentration of human iNOS holoenzyme is 1.5 nM.

b

The solution viscosity with the added 7 % Ficoll 70 or 4.6 % Dextran 70 is the same (2.7 cP).

c

The solution viscosity with the added 14 % Ficoll 70 or 7.5% Dextran 70 is the same (5.0 cP).