Figure 6.

MyD88 signaling in radio-sensitive/bone marrow-derived cells but not radio-resistant cells contributes to the production of RV-specific IgG and IgA. (A) Bone marrow chimeric mice were made where WT mice reconstituted with MyD88 KO bone marrow. After rest and confirmation of chimerism, experimental and control mice were inoculated with 10⁵ SD₅₀ murine RV and bled until 9 wk PI. (A) Serum was probed for anti-RV IgG production and (B) IgG titer. The same serum was also probed for RV-specific (C) IgA production and (D) IgA titer. (E) Another group of bone marrow chimeric mice were made where MyD88 KO mice were reconstituted with WT bone marrow. Mice were treated as in (A–C), and serum was probed for anti-RV IgG and (F) IgA production. Results in (A–F) are from an individual experiment (n = 4–5) with bars or horizontal lines representing the mean and error bars denoting the SEM. *P < 0.05.