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. 2020 Aug 19;10(10):3775–3782. doi: 10.1534/g3.120.401400

Table 2. In-situ assembly & integration efficiency.

Hygromycin Resistant Broods In-situ Assembly
Marker Positive Broods All Roller Mixed All wt Homozygous Roller Isolated PCR Confirmed Integrations Error Free Integrations
Plasmid 15 3 0 0 3 3 3 (20.0%)
2pc PCR 41 6 3 0 8 6 3 (7.3%)
6pc PCR 51 0 8 5 1 1 0 (0.0%)