Table 2. In-situ assembly & integration efficiency.
Hygromycin Resistant Broods | In-situ Assembly | ||||||
---|---|---|---|---|---|---|---|
Marker Positive Broods | All Roller | Mixed | All wt | Homozygous Roller Isolated | PCR Confirmed Integrations | Error Free Integrations | |
Plasmid | 15 | 3 | 0 | 0 | 3 | 3 | 3 (20.0%) |
2pc PCR | 41 | 6 | 3 | 0 | 8 | 6 | 3 (7.3%) |
6pc PCR | 51 | 0 | 8 | 5 | 1 | 1 | 0 (0.0%) |