Figure 1.

Sample and data collection scheme. The figure shows the cross of a single male and female mosquito to produce female F1 offspring. The F0 father and mother were sequenced individually using Illumina HiSeq to obtain short reads. Genomic DNA from a pool of the female F1 sibling was sequenced using three Oxford Nanopore MinION flow cells to produce long reads. Genomic DNA from a pool of the paternal half-sibling F1 males was used to generate Bionano DLS optical mapping. Oxford Nanopore reads were used to generate the contigs using Canu (Koren et al., 2017) and the Canu contigs were polished using the parental Illumina reads. The polished Canu contigs were scaffolded using the Bionano DLS optical map to generate the AalbS3 assembly.